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Thu. Nov 30th, 2023

Phage show is without doubt one of the strongest and broadly used laboratory method for the examine of protein-protein, protein-peptide and protein-DNA interactions. This expertise is especially based mostly on displaying the curiosity protein (peptides, antibodies, scaffolds or others) on the floor of using phage after which be used to interrogate the constructed libraries containing thousands and thousands and even billions of displayed phages. Theoretically, phage show is an exogenous gene expression technique which the gene encoding the curiosity protein is inserted into bacteriophage coat protein gene then displaying the curiosity protein on the phage surfaces, leading to a connection between genotype and phenotype.

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