Library Building and Screening
Fig.1 Workflow of phage show building
and screening (Díez et al. 2015)
Phage show is among the strongest and broadly used laboratory approach for the examine of protein-protein, protein-peptide and protein-DNA interactions. This know-how is principally primarily based on displaying the curiosity protein (peptides, antibodies, scaffolds or others) on the floor of using phage after which be used to interrogate the constructed libraries containing tens of millions and even billions of displayed phages. Theoretically, phage show is an exogenous gene expression methodology which the gene encoding the curiosity protein is inserted into bacteriophage coat protein gene then displaying the curiosity protein on the phage surfaces, leading to a connection between genotype and phenotype.